Bio-Rad CFX Connect RT-PCR System

 cfxconnect

 

Bio-Rad CFX Connect RT-PCR system:
  • excitation/emission 450-580 nm
  • temperature range 0-100 °C with max.ramp 5 °C/sec
  • RT-PCR and extrinsic-DSF capable 
  • universal SYBR Green and SYBR Orange compatible
  • Low sample volumes for DSF with high throughput (96-well plate read/hour)
  • Protein stability dependent on solution (Tm)
  • PCCF developped stability plate with varying pH and salt concentration
  • CFX Maestro Software

Extrinsic DSF 100 kDa protein, pI=5.94

Using PCCF developped stability plate with varying range of pH and salt concentration
melt