How to acknowledge PCCF in publication

Research described in this paper was performed in part at the Protein Characterization and Crystallization Facility (PCCF), which is supported by the College of Medicine, University of Saskatchewan, Saskatoon, Canada.



MEMO1 binds iron and modulates iron homeostasis in cancer cells
Dolgova, N., Uhlemann, Eva-Maria., Boniecki, M.T., Vizeacoumar, F.S., Ara, A., Nouri, P., Ralle, M., Tonelli, M., Abbas, S.A., Patry, J., Elhasasna, H., Freywald, A., Vizeacoumar, F., Dmitriev, O.Y.,  eLIFE 2024


A Neglected and Emerging Antimicrobial Resistance Gene Encodes for a Serine-Dependent Macrolide Esterase
Dhindwal, P., Thompson, C., Kos, D., Planedin, K., Jain, R., Jelinski, M., Ruzzini, A., PNAS 2023


Targeting of Microvillus Protein Eps8 by the NleH Effectors Kinases from Enteropathogenic E.coli
Pollock, G.L., Grishin, A.M., Giogha, C., Gan, J., Oates, C.V., McMillan, P.J., Gaeta, I., Tyska, M.J., Pearson, J.S., Scott, N.E., Cygler, M., Hartland, E.L., PNAS 2022
Structural Study of Legionella pneumophila DotY (Lpg0294), a component of the Dot/Icm type IV Secretion System
Chung, IYW, Cygler, M. Acta Crystallogr F Struct Biol Commun 2022


Structural and functional characterization of Legionella  pneumophila effector MavL
Voth, K., Pasricha, S., Chung, I.Y.W., Wibawa, R.R., Zainudin, E.N.H.E., Hartland, E.L., Cygler, M. Biomolecules 2021


Legionella effector LegA15/AnkH contains an unrecognized cysteine protease-like domain and displays structural similarity to LegA3/AnkD, but differs in host cell localization
Chung IYW, Lei Li, Cygler M. Acta Crystallogr D Structure Biol 2021

N-terminal tyrosine of ISCU2 triggers [2Fe-2S] cluster synthesis by ISCU2 dimerization
Freibert SA, Boniecki MT, Stumpfig C, Schultz V, Krapoth N, Winge DR, Muhlenhoff U, Stehling O, Cygler M, Lill R. Nat Commun 2021

Disulfide Bonds Play a Critical Role in the Structure ad Function of the Receptor-binding Domain of the SARS-CoV-2 Spike Antigen
Grishin AM, Dolgova NV, Landreth S, Fisette O, Pickering IJ, George GN, Falzarano D, Cygler M. J Mol Biol 2021

Structural and functional study of Legionella pneumophila effector RavA.
Chung IYW, Li L, Tyurin O, Gagarinova A, Wibawa R, Li P, Hartland EL, Cygler M. Protein Science 2021


Cell-Penetrating Antimicrobial Peptides Derived from an Atypical Staphylococcal d-Toxin
Deeyagahage K., Ruzzini A. Microbial Spectr 2021


Kinetic and Structural analysis of Escherichia coli phosphoenolpyruvate carboxykinase mutants.
Sokaribo, A., Novakovski, B.A.A., Cotelesage, J., White, A.P., Sanders, D.A.R., Goldie, H.,
BBA Gen Subj 2020
Nanobodies against the metal binding domains of ATP7B as tools to study copper transport in the cell.
Uhlemann, E-M.E., Corey Y.H., Patry, J., Dolgova, N., Lutsenko, S., Muyldermans, S., Dmitriev, O.Y.
Metallomics 2020
Identification and Mechanistic Characterization of a Peptide Inhibitor of Glycogen Synthase Kinase (GSK3B) Derived from the Disrupted in Schizophrenia 1 (DISC1) Protein.
Saundh, S.L., Patnaik, D., Gagne, S., Bishop, J.A., Lipsit, S., Amat, S., Pujari, N., Nambisan, A.K., Bigsby, R., Murphy, M., Tsai, L-H., Haggarty, S.J., Leung, A. K-W.
ACS Chem Neuroscience 2020

Asparagine-84, a regulatory allosteric site residue, helps maintain the quaternary structure of Campylobacter jejuni dihydrdipicolinate synthase.
Yazdi, M.M., Saran, S., Mrozowich, T., Lehnert, C., Patel, T.R., Sanders D.A.R., Palmer, D.R.J.
Journal of Structural Biology 2020

An Indispensable Role for the MavE Effector of Legionella pneumophila in Lysosomal Evasion.
Vaughn B, Voth K, Price CT, Jones S, Ozanic M, Santic M, Cygler M, Abu Kwaik Y.
mBIO 2020
The Legionella kinase LegK7 exploits the Hippo pathway scaffold protein MOB1A for allostery and substrate phosphorylation.
Lee PC, Beyrakhova K, Xu C, Boniecki MT, Lee MH, Onu CJ, Grishin AM, Machner MP, Cygler M.
PNAS 2020



Structure and Function of the putative thioredoxin 1 from the thermophilic eubacterium Thermosipho africanus strain TCF52B.
Sahtout, N., Kuttiyatveetil, J.R.A., Sanders, D.A.R.
BBA Proteins and Proteomics 2019
Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex.
Von Dwingelo J, Chung IYW, Price CT, Li L, Jones S, Cygler M, Abu Kwaik Y.
mBio 2019
Salmonella Effectors SseK1 and SseK3 Target Death Domain Proteins in the TNF and TRAIL Signaling Pathways.
Newson JPM, Scott NE, Yeuk Wah Chung I, Wong Fok Lung T, Giogha C, Gan J, Wang N, Strugnell RA, Brown NF, Cygler M, Pearson JS, Hartland EL.
Mol Cell Proteomics 2019
The structure of Legionella effector protein LpnE provides insights into its interaction with Oculocerebrorenal syndrome of Lowe (OCRL) protein.
Voth KA, Chung IYW, van Straaten K, Li L, Boniecki MT, Cygler M.
FEBS J 2019

Structure of Ddi2, a highly inducible detoxifying metalloenzyme from S. cerevisiae
Li, J., Jia, Y., Lin, A., Hanna, M., Chelico, L., Xiao, W., and Moore S. A.
JBC 2019

Patient-derived mutations within the N- terminal domains of p85alpha impact PTEN or Rab5 binding and regulation.
Mellor P, Marshall JDS, Ruan X, Whitecross DE, Ross RL, Knowles MA, Moore SA, Anderson DH.
Scientific Reports 2019


Legionella pneumophila effector Lem4 is a membrane-associated protein tyrosine phosphatase.
Beyrakhova K, Li L, Xu C, Gagarinova A, Cygler M.
JBC 2018
Structural and functional characterization of PL28 family ulvan lyase NLR48 from Nonlabens ulvanivorans.
Ulaganathan T, Banin E, Helbert W, Cygler M.
JBC 2018
Regulation of Shigella Effector Kinase OspG through Modulation of Its Dynamic Properties.
Grishin AM, Barber KR, Gu RX, Tieleman DP, Shaw GS, Cygler M.
JMB 2018
Structure-function analyses of a PL24 family ulvan lyase reveal key features and suggest its catalytic mechanism.
Ulaganathan T, Helbert W, Kopel M, Banin E, Cygler M.
JBC 2018
Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain
Marshal, J.D.S., Mellor, P., Ruan, X., Whitecross, D.E., Moore, S.A., Anderson, D.H.,
Oncotarget 2018



Structure and functional dynamics of the mitochondrial Fe/S cluster synthesis complex.
Boniecki MT, Freibert SA, Mühlenhoff U, Lill R, Cygler M.
Nat.Commun 2017
Molecular basis for the binding and modulation of V-ATPase by a bacterial effector protein.
Zhao J, Beyrakhova K, Liu Y, Alvarez CP, Bueler SA, Xu L, Xu C, Boniecki MT, Kanelis V, Luo ZQ, Cygler M, Rubinstein JL.
PLOS 2017
New Ulvan-Degrading Polysaccharide Lyase Family: Structure and Catalytic Mechanism Suggests Convergent Evolution of Active Site Architecture.
Ulaganathan T, Boniecki MT, Foran E, Buravenkov V, Mizrachi N, Banin E, Helbert W, Cygler M.
ACS Chem Biol 2017
Conformational flexibility of PL12 family heparinases: structure and substrate specificity of heparinase III from Bacteroides thetaiotaomicron (BT4657).
Ulaganathan T, Shi R, Yao D, Gu RX, Garron ML, Cherney M, Tieleman DP, Sterner E, Li G, Li L, Linhardt RJ, Cygler M.
Glycobiology 2017


Biochemical characterization of INTS3 and C9ORF80, two subunits of hNABP1/2 heterotrimeric complex in nucleic acid binding.
Vidhyasagar V, He Y, Guo M, Talwar T, Singh RS, Yadav M, Katselis G, Vizeacoumar FJ, Lukong E, Wu Y.
Biochem J., 2017


The DEAD-box Protein DDX43 (HAGE) Is a Dual RNA-DNA Helicase and Has a K-homology Domain Required for Full Nucleic Acid Unwinding Activity.
Talwar,T., Vidhyasagar,V., Qing,J., Guo,M., Kariem,A., Lu,Y., Singh,RS., Lukong,KE., and Wu, Y.
JBC 2017



Structural and Functional Investigations of the Effector Protein LpiR1 from Legionella pneumophila.
Beyrakhova KA, van Straaten K, Li L, Boniecki MT, Anderson DH, Cygler M.
JBC 2016


Structural basis for the inhibition of host protein ubiquitination by Shigella effector kinase OspG.
Grishin AM, Condos TE, Barber KR, Campbell-Valois FX, Parsot C, Shaw GS, Cygler M.
Structure 2014
NleH defines a new family of bacterial effector kinases.
Grishin AM, Cherney M, Anderson DH, Phanse S, Babu M, Cygler M.
Structure 2014