NanoTemper Monolith NT.115
The PCCF Monolith NT.115 is a BLUE/RED model (ex.493 nm / 650 nm and em. 521 nm / 670 nm)
Monolith NT.115 is employing Microscale Thermophoresis (MST) method to detect binding between molecules.
MST technique measures binding between two molecules by detecting variation in the fluorescense signal of a labeled target as a result of an IR-laser induced temperature changes.
The MST method is an easy, fast and precise approach to quantify biomolecular interactions:
detects binding affinity in range between 1 nM - 500 mM
binding of ligands between 40 Da and 2.5 MDa in size
independent of solution used (from standard buffer to complex fluids including cell lysates and blood)
requires 1 - 10 nM labeled molecule and dilutions of ligand (starting at 20 fold concentration above expected dissociation constant)
4 µl of sample loaded into max. of 16 capillaries